#NephJC Transcript
Healthcare social media transcript of the #NephJC hashtag.
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See #NephJC Influencers/Analytics.
| Profile | Tweet |
|---|---|
 | Nephrology Journal Club @NephJC Welcome to tonight’s basic/translational #NephJC discussion! It’s 9 pm EST, so let’s get started. #NephJC |
 | nyiminhan @nyiminhan RT @NephJC: Welcome to tonight’s basic/translational #NephJC discussion! It’s 9 pm EST, so let’s get started. #NephJC |
 | Matthew Sparks, MD @Nephro_Sparks Matt Sparks reporting for duty #BasicScience #Translational #NephJC nights are my holidays. No COI except --> really excited. Thanks @DvelaMoran 4 joining @VanessaLerma17 4 summary @SusieLMurray 4 editorial & @MarvinGonzlez16 4 hosting also @jenniejlin and @HecmagsMD for edits |
| Nephrology Journal Club @NephJC Tonight we are discussing the latest paper from @AnnaGreka lab: discovery of a novel therapeutic strategy for treating Mucin 1 kidney disease #NephJC |
 | Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @Nephro_Sparks: Matt Sparks reporting for duty #BasicScience #Translational #NephJC nights are my holidays. No COI except --> really excited. Thanks @DvelaMoran 4 joining @VanessaLerma17 4 summary @SusieLMurray 4 editorial & @MarvinGonzlez16 4 hosting also @jenniejlin and @HecmagsMD for edits |
 | Joel M. Topf, MD FACP @kidney_boy Joel Topf, total clinician here. You can tell because I'm behind on my charts. Will be checking in from time to time while I catch up. #NephJC |
 | Michelle Rheault @rheault_m @NephJC Hi. Michelle here. Looking forward to learning something. #nephjc |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @NephJC: Tonight we are discussing the latest paper from @AnnaGreka lab: Tonight we are discussing the latest paper from @AnnaGreka lab: discovery of a novel therapeutic strategy for treating Mucin 1 kidney disease #NephJC |
 | Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD Hector Madariaga. Hi, everyone! No COI. From #PatriotsNation #Nephjc https://t.co/osiok5kscp |
| Nephrology Journal Club @NephJC Everyone, please introduce yourself and declare any relevant conflicts of interest (COIs). Don’t forget the hashtag #NephJC |
| Nephrology Journal Club @NephJC I’m Marvin Gonzalez, @MarvinGonzlez16 from @CistaNicaragua @RenalUCL, and your host for tonight’s chat. I’m also #NSMC Class of 2019. No COI. #NephJC |
 | Vanessa Lerma @VanessaLerma17 Hi Everyone. I'm Vanessa from Mexico. Ready for discussion tonight. #pod8 #NephJC |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil Swapnil Hiremath, clinical nephrologist from @ottawarenal No CoI #NephJC cofounder Totally ignorant on this topic: here to learn https://t.co/a3D87f27XW |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD RT @NephJC: I’m Marvin Gonzalez, @MarvinGonzlez16 from @CistaNicaragua @RenalUCL, and your host for tonight’s chat. I’m also #NSMC Class of 2019. No COI. #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @rheault_m @NephJC Hi Michelle. What wine did you get? 🍷#NephJC |
 | Samira Farouk, MD, MSCR @ssfarouk Samira, transplant nephrologist @ISMMSKidney. Ready to learn about MUC-1 #NephJC |
 | Jennie Lin 👩🏻⚕️🧬 @jenniejlin Jennie Lin, @NU_Nephrology physician-scientist, reporting for duty from my office! All you nephrons are welcome to visit. No COI. #NephJC https://t.co/wuh08cDr3Q |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @kidney_boy Can you dictate your notes? Or you type? #NephJC |
| Nephrology Journal Club @NephJC If you haven’t yet, please check out the summary from @VanessaLerma17 https://t.co/ub1j0WBd0q #NephJC |
| Nephrology Journal Club @NephJC Here is the #VisualAbstract from MUC1 kidney disease…..#NephJC @VanessaLerma17 https://t.co/KUi9ibdciQ |
 | Joshua Waitzman, MD PhD @Jwaitz @NephJC Hi! Josh Waitzman, research fellow at @BIDMChealth here. Loved this paper! No COI #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @VanessaLerma17 Hi Vanessa! Hope all is well #NephJC |
| Joel M. Topf, MD FACP @kidney_boy Venessa wrote the summary on NephJC for tonight's discussion, so if you don't understand any part of the study just ask her. #NephJC |
 | Moran Dvela-Levitt @DvelaMoran Hi everyone! thank you for having me! this is my first time at #NephJC so I hope to keep track of everything! |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @NephJC: Here is the #VisualAbstract from MUC1 kidney disease…..#NephJC @VanessaLerma17 https://t.co/KUi9ibdciQ |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @NephJC: If you haven’t yet, please check out the summary from @VanessaLerma17 https://t.co/ub1j0WBd0q #NephJC |
 | Tiffany Caza @Tiff_Caza RT @NephJC: Here is the #VisualAbstract from MUC1 kidney disease…..#NephJC @VanessaLerma17 https://t.co/KUi9ibdciQ |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil Author in the house! Welcome to #NephJC |
| Samira Farouk, MD, MSCR @ssfarouk If you didn't have time to read the paper, scroll through @Nephro_Sparks #tweetorial #NephJC https://t.co/1liGJQ0CUc |
| Nephrology Journal Club @NephJC Tonight we have the pleasure of having the first author joining us for the discussion! Please welcome @DvelaMoran. Congratulations on the paper! #NephJC |
 | L. Parker Gregg @LParkerGregg1 Parker, nephrologist in Dallas. No COI. Looking forward to learning about something I know nothing about! #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD Am I the only one who had to read the paper several times to understand it? I am here to learn. #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @ssfarouk @ISMMSKidney Hi Samira #NephJC |
 | Sami Alasfar, MD, MPH, FASN @SamiAlasfar Transplant Nephrologist here. Representing Hopkins. No related OCI. #NephJC |
| Joshua Waitzman, MD PhD @Jwaitz @DvelaMoran Hi @DvelaMoran! Gorgeous work here! #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @NephJC @DvelaMoran Welcome, @DvelaMoran and congrats on your tour de force of a paper! #NephJC |
| Moran Dvela-Levitt @DvelaMoran @NephJC It's a great pleasure! thank you #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @DvelaMoran Awesome. So happy to have you. Welcome! #NephJC |
| Tiffany Caza @Tiff_Caza @NephJC Hi everyone! I'm Tiff. I'm a #NSMC intern, member of pod ⚡, and lover of basic science. No COI. Really excited for this one! #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @DvelaMoran Thanks for joining @DvelaMoran excited to have you #NephJC |
| Matthew Sparks, MD @Nephro_Sparks RT @DvelaMoran: Hi everyone! thank you for having me! this is my first time at #NephJC so I hope to keep track of everything! |
| Matthew Sparks, MD @Nephro_Sparks @Tiff_Caza @NephJC Hi Tiff. how are you? #NephJC |
| Nephrology Journal Club @NephJC Link to paper: DOI: https://t.co/KCfmFsu4Yy #NephJC |
 | Silvi Shah, MD, MS @silvishah Hi this is Silvi from UCincy no coi #nephJC |
| Joel M. Topf, MD FACP @kidney_boy @HecmagsMD I'm a very good typer #NephJC https://t.co/bzanObyZmY |
 | Airawat ऐरावत @Renaltubules Ashwasena. coi: loves peds Nephro #NephJC |
| Nephrology Journal Club @NephJC Abbreviations: ADTKD: Autosomal Dominant Tubulointerstitial Kidney Disease, iPSC: induced pluripotent stem cell MUC1: Mucin-1, MUC1-fs: Mucin-1 misfolded, MKD: Mucin-1 Kidney Disease UPR: unfolded protein response #NephJC |
| Samira Farouk, MD, MSCR @ssfarouk From the #NephJC summary MKD = MUC-1 kidney disease Mutations in the MUC1 gene (on chromosome 1q21) have been identified to cause progressive tubulointerstitial kidney disease inherited in an autosomal dominant fashion and thus now termed, MKD |
| Joel M. Topf, MD FACP @kidney_boy Am I the only one here who skipped the paper and just read Matt's Tweetorial two times? #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @Tiff_Caza @NephJC I'm ok. The day has finally come #BasicScience #NephJC night. love it |
 | Marvin Gonzalez. MD, Ph.D @MarvinGonzlez16 You are not the only one, me too #NephJc |
 | Beje Thomas @bthomas215 Hey Beje from Georgetown no COI #nephjc |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @NephJC: Abbreviations: ADTKD: Autosomal Dominant Tubulointerstitial Kidney Disease, iPSC: induced pluripotent stem cell MUC1: Abbreviations: ADTKD: Autosomal Dominant Tubulointerstitial Kidney Disease, iPSC: induced pluripotent stem cell MUC1: Mucin-1, MUC1-fs: Mucin-1 misfolded, MKD: Mucin-1 Kidney Disease UPR: unfolded protein response #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @kidney_boy There's so much cool stuff in the paper though. Worth reading in depth. #NephJC |
 | Mario Funes, MD @MarioFunesMD Hello everyone. Mario Funes, PGY3 IM in New Brunswick, NJ. #NSMC intern 2019. No COI. #NephJC |
| Vanessa Lerma @VanessaLerma17 RT @kidney_boy: Am I the only one here who skipped the paper and just read Matt's Tweetorial two times? #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @bthomas215 Beje!!! My bro!!! :) Good to see you here #NephJC |
| Matthew Sparks, MD @Nephro_Sparks Have to plug our free pre meeting that will occur Wed before the kidney meeting in DC #NephJC it will be a full day of #BasicScience #TranslationalScience Do it now!!!!!!!!!!! https://t.co/35aFfKiXCo |
| Sami Alasfar, MD, MPH, FASN @SamiAlasfar RT @NephJC: Abbreviations: ADTKD: Autosomal Dominant Tubulointerstitial Kidney Disease, iPSC: induced pluripotent stem cell MUC1: Abbreviations: ADTKD: Autosomal Dominant Tubulointerstitial Kidney Disease, iPSC: induced pluripotent stem cell MUC1: Mucin-1, MUC1-fs: Mucin-1 misfolded, MKD: Mucin-1 Kidney Disease UPR: unfolded protein response #NephJC |
| Joshua Waitzman, MD PhD @Jwaitz @Nephro_Sparks @DvelaMoran Christmas or #basicscience night? #nephjc https://t.co/evL077oJJI |
 | Anna Greka @AnnaGreka @DvelaMoran Happy to serve as backup, @DvelaMoran is on top of everything, lurking in the background, happy to be here and delighted and honored you are discussing our work #nephjc |
| Nephrology Journal Club @NephJC Here we go ... Let’s get into it with some introductory questions. #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin For the experimental scientists out there, any tips for how folks should approach reading a basic science paper? I like abstract/intro, then methods, then combing through figures. #NephJC |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @Nephro_Sparks: Have to plug our free pre meeting that will occur Wed before the kidney meeting in DC #NephJC it will be a full day of #BasicScience #TranslationalScience Do it now!!!!!!!!!!! https://t.co/35aFfKiXCo |
| Samira Farouk, MD, MSCR @ssfarouk RT @Nephro_Sparks: Have to plug our free pre meeting that will occur Wed before the kidney meeting in DC #NephJC it will be a full day of #BasicScience #TranslationalScience Do it now!!!!!!!!!!! https://t.co/35aFfKiXCo |
| Matthew Sparks, MD @Nephro_Sparks @AnnaGreka @DvelaMoran Hi Anna. Thanks for coming #NephJC |
| Nephrology Journal Club @NephJC T0a: Do you follow any patients with tubulointerstitial kidney disease? If so, how were they diagnosed? #NephJC |
 | Justin Davis @jbda19 RT @NephJC: Here is the #VisualAbstract from MUC1 kidney disease…..#NephJC @VanessaLerma17 https://t.co/KUi9ibdciQ |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @jenniejlin Good question #Nephjc https://t.co/Tb7UHMdX1H |
 | Susan Murray @SusieLMurray Susie Murray, RCSI In Dublin. No COI #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Nephro_Sparks And it counts on your CV as an oral abstract presentation if selected - odds are forever in your favor for a small forum like this one. #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @SusieLMurray Hi Susie. Welcome! Your commentary was awesome #NephJC |
| Beje Thomas @bthomas215 Very nice VA #nephjc |
| Matthew Sparks, MD @Nephro_Sparks @jenniejlin Im hitting the figures and the methods first. #NephJC |
| Joel M. Topf, MD FACP @kidney_boy @HecmagsMD #NewSchool doctors look at me and say he's #OldSchool and #OldSchool doctors look at me and say he's #NewSchool Which makes me a perfect GenX'er #NephJC |
| Justin Davis @jbda19 @NephJC Justin #NSMC intern. Finishing up transplant clinic and curious to learn more about ADTKD related pathology. I suspect we miss a few of these diagnoses for our younger CKD cohort. #nephjc |
| Nephrology Journal Club @NephJC RT @AnnaGreka: @DvelaMoran Happy to serve as backup, @DvelaMoran is on top of everything, lurking in the background, happy to be here and delighted and honored you are discussing our work #nephjc |
| Joshua Waitzman, MD PhD @Jwaitz @NephJC It's the classic "probably not sick enough to treat, so probably don't biopsy" disease, so my guess is that we all see a lot of this, but don't diagnose it often enough. #NephJC |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil @NephJC I probably do follow some/many patients with ADTKD. But I don’t know it, and neither do they 😕 #NephJC |
| Nephrology Journal Club @NephJC T0b: What do we know about the clinical presentation of ADTKD? #NephJC |
| Nephrology Journal Club @NephJC T0c: Here is a new review on how ADTKD is classified #NephJC https://t.co/LigEA3UK1z https://t.co/gM1nwhoHgK |
| Michelle Rheault @rheault_m @jenniejlin I like to read the intro then discussion. Then go back to methods and figures to see if authors really did what they said/think they did. #nephjc |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Jwaitz @NephJC Especially if incidentally also have T2DM #NephJC |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil @SusieLMurray Whoah. What tine is it there? And thanks for that excellent commentary! #NepHJC |
 | Gates Colbert, MD @DoctorGates Dallas Nephrologist. Lurking tonight from the smarty pants of the specialty. #NephJC |
| Joshua Waitzman, MD PhD @Jwaitz @jenniejlin @NephJC Did I hear SGLT2i deficiency? #NephJC |
 | Katalin Susztak @KSusztak @jenniejlin Katalin Susztak here for #NephJC for me figures are everything ! Methods if it is new method paper |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @NephJC: T0c: T0c: Here is a new review on how ADTKD is classified #NephJC https://t.co/LigEA3UK1z https://t.co/gM1nwhoHgK |
| Sami Alasfar, MD, MPH, FASN @SamiAlasfar RT @NephJC: T0c: T0c: Here is a new review on how ADTKD is classified #NephJC https://t.co/LigEA3UK1z https://t.co/gM1nwhoHgK |
| Matthew Sparks, MD @Nephro_Sparks @SusieLMurray Hi Susan thanks for coming and your great summary #NephJC |
| Nephrology Journal Club @NephJC T0d: @Nephro_Sparks provided a great summary of the genetic and protein abnormalities seen in MUC1 in MKD https://t.co/QwhvBcc4hy #NephJC |
 | Jenn @Jentan7477 RT @Nephro_Sparks: Have to plug our free pre meeting that will occur Wed before the kidney meeting in DC #NephJC it will be a full day of #BasicScience #TranslationalScience Do it now!!!!!!!!!!! https://t.co/35aFfKiXCo |
| Joel M. Topf, MD FACP @kidney_boy This is what I think and would have said if I was a faster thinker and a faster typer. #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @NephJC Young patients with elevated creatinine. Usually kidneys look OK in US. Bland urine sediment. No hematuria. + family history. No compelling reasons to biopsy... #NephJC |
| Nephrology Journal Club @NephJC T0e: What is your approach to treating patients with ADTKD #NephJC |
| Vanessa Lerma @VanessaLerma17 RT @NephJC: T0c: T0c: Here is a new review on how ADTKD is classified #NephJC https://t.co/LigEA3UK1z https://t.co/gM1nwhoHgK |
| Joshua Waitzman, MD PhD @Jwaitz @kidney_boy Thanks @kidney_boy #nephjc https://t.co/gcGMelKyeD |
| Matthew Sparks, MD @Nephro_Sparks @NephJC currently we have no specific therapy available. Things could be changing if we start to study these #research more @NIH funding. #NephJC |
| Moran Dvela-Levitt @DvelaMoran @NephJC This is one of the major challenges like in many kidney diseases.. no actual therapies are available #NephJC |
| Joel M. Topf, MD FACP @kidney_boy @NephJC You don't have to treat it if you never make the diagnosis #NephJC https://t.co/v2MzuZkMTL |
| Nephrology Journal Club @NephJC T1: Let’s move on to the Methods section. #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @NephJC my favorite section #NephJC |
 | Ralph Nader, MD, FASN @Ralph__Nader RT @NephJC: T0c: T0c: Here is a new review on how ADTKD is classified #NephJC https://t.co/LigEA3UK1z https://t.co/gM1nwhoHgK |
| Nephrology Journal Club @NephJC T1a: Mechanisms of MKD were studied using complementary models. These included mouse models for phenotype/pharmacology, 2D cell culture for molecular bio, and 3D kidney organoid culture for translational proof of principle. #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @NephJC @Nephro_Sparks is ready to unleash about the mouse models! #NephJC 🐁🐁🐁🐁🐁 |
| Joshua Waitzman, MD PhD @Jwaitz @NephJC How many mouse models was that @Nephro_Sparks? #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @NephJC Conservative management. But hopefully after the findings of this paper, we will have a therapeutic intervention! :) #Hopeful #NephJC |
| Nephrology Journal Club @NephJC T1b: 3 different mouse models were generated: mMuc1wt(+) mouse normal Muc1 hMUC1wt(wt) human normal MUC1 hMUC1fs(fs) human mutant MUC1fs https://t.co/xgoQZ6R9Ex #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @NephJC love this approach. Really just a great approach to combine mouse models with human cell culture and organoids. Could lead to quicker translation to humans #NephJC |
| Tiffany Caza @Tiff_Caza @HecmagsMD @NephJC And still hard to diagnose if you choose to biopsy 😕. Non-specific injury with tubulointerstitial damage > glomerular and vascular scarring. #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @kidney_boy @NephJC How would you make a diagnosis? Biopsy? Genetic testing? #NephJC |
| Moran Dvela-Levitt @DvelaMoran @HecmagsMD @NephJC This is definitely our big hope @HecmagsMD #NephJC |
| Nephrology Journal Club @NephJC RT @NephJC: T1b: T1b: 3 different mouse models were generated: mMuc1wt(+) mouse normal Muc1 hMUC1wt(wt) human normal MUC1 hMUC1fs(fs) human mutant MUC1fs https://t.co/xgoQZ6R9Ex #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @NephJC love this approach. need to ensure the human WT does not cause disease in mouse model. #NephJC |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @HecmagsMD: @NephJC Young patients with elevated creatinine. Usually kidneys look OK in US. Bland urine sediment. No hematuria. + family history. No compelling reasons to biopsy... #NephJC |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @NephJC: T1b: T1b: 3 different mouse models were generated: mMuc1wt(+) mouse normal Muc1 hMUC1wt(wt) human normal MUC1 hMUC1fs(fs) human mutant MUC1fs https://t.co/xgoQZ6R9Ex #NephJC |
| Nephrology Journal Club @NephJC T1c: Immortalized kidney epithelial cell lines were generated from a control patient (N cells) and a MKD patient (P cells). Kidney tissue from these patients were dissociated and immortalized with hTERT lentivirus. #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @Nephro_Sparks @NephJC Obviously! #Nephjc https://t.co/4fDlllqCKm |
 | devika nair md msci @devimol @NephJC such a thoughtful approach: investigators found that TMED9 co-localized in same compartments as MUC1 + was highly prevalent in organoid cells expressing MUC1, so TMED9 must have an important clinical implication #NephJC |
| Moran Dvela-Levitt @DvelaMoran @Tiff_Caza @HecmagsMD @NephJC That's true, but if there is a family history it's worth sending us a sample to @broadinstitute to have the only CLIA assay for the most common C insertion #NephJC |
| Joel M. Topf, MD FACP @kidney_boy RT @Tiff_Caza: @HecmagsMD @NephJC And still hard to diagnose if you choose to biopsy 😕. Non-specific injury with tubulointerstitial damage > glomerular and vascular scarring. #NephJC |
| Nephrology Journal Club @NephJC T1d: Immortalized N cells and P cells were used for downstream studies, including gene expression profiling (RNA-seq), apoptosis assays, immunoblots, small molecule screening, and thermal shift assays. #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @NephJC Patient engagement was very key here. Getting actual tissue from patient and healthy siblings is something I have not seen before. Impressed #NephJC |
| Joel M. Topf, MD FACP @kidney_boy @DvelaMoran @Tiff_Caza @HecmagsMD @NephJC @broadinstitute and then what? What do you do with that information? #NephJC |
| Moran Dvela-Levitt @DvelaMoran @HecmagsMD @kidney_boy @NephJC at @broadinstitute we have a CLIA assay to identify the most common C insertion mutation #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @NephJC 👆This is an important point - currently difficult to execute some of these more mechanistic studies in 3D organoid culture, but important to demonstrate proof-of-principe in the organoid for translation. #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @DvelaMoran @Tiff_Caza @NephJC @broadinstitute How does the work for a patient living in, let’s say, California? Can you elaborate on how patients could send a blood sample? Or who to contact? #NephJC |
| Nephrology Journal Club @NephJC T1e: Kidney organoids were differentiated from iPSCs of healthy and MKD patients (Takasato protocol) and stained for protein localization and proof of principle pharmacology studies. #NephJC |
| Michelle Rheault @rheault_m @kidney_boy @DvelaMoran @Tiff_Caza @HecmagsMD @NephJC @broadinstitute Can answer question of “Why do I have kidney disease” and identify other at risk family members for screening. #nephjc |
| Samira Farouk, MD, MSCR @ssfarouk @Nephro_Sparks @NephJC I'm sure was challenging to consent healthy siblings for kidney biopsy #NephJC |
| Moran Dvela-Levitt @DvelaMoran @kidney_boy @Tiff_Caza @HecmagsMD @NephJC @broadinstitute The information by itself is very valuable as you can imagine in regard to kidney transplantation from a family member etc. #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @kidney_boy @DvelaMoran @Tiff_Caza @HecmagsMD @NephJC @broadinstitute the more families identified with mutation the quicker we can start to build cohorts and have them enroll in clinical trials. We have to start building these or we will continue to say this #NephJC #TheFutureIsNow |
| Joshua Waitzman, MD PhD @Jwaitz @devimol @NephJC Question for @AnnaGreka and @DvelaMoran--how much did you know about TMED9 before starting this work? Did you go in suspecting this pathway or something else? #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @NephJC @DvelaMoran did the organoids differentiate with normal nephron structures from both N and P iPSC lines? #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @kidney_boy @DvelaMoran @Tiff_Caza @NephJC @broadinstitute I guess this could lead to other family members to be screened and be educated about it. Many patients don’t have an answer about their kidney disease. And now, we may have a potential treatment…? #NephJC |
| Moran Dvela-Levitt @DvelaMoran @Jwaitz @devimol @NephJC @AnnaGreka While starting this study we didn't know much about TMED9 and definitely did not originally suspect it's connection to this disease. most reasonable hypothesis was ER accumulation and ER stress #NephJC |
| Nephrology Journal Club @NephJC T1f: Any thoughts on this general approach? More methods will be further explained as needed when discussing results. #NephJC |
| Joel M. Topf, MD FACP @kidney_boy None of the other authors answer this question. This question is just for Anna and Dvela. You're welcome Josh. |
| Vanessa Lerma @VanessaLerma17 @NephJC What is your opinion about the results found in female vs male mice?#nephjc |
| Joshua Waitzman, MD PhD @Jwaitz @kidney_boy That's the power of the bully pulpit. @kidney_boy speaks and I get answers! (but seriously, thanks @DvelaMoran for your thoughtful reply) #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @Nephro_Sparks @kidney_boy @DvelaMoran @Tiff_Caza @NephJC @broadinstitute This is the type of research that Dr Bleyer at Wake Forest works on. He has identified several families carrying this diagnosis #NephJC |
| Nephrology Journal Club @NephJC Now it’s time to discuss the results of this interesting study. #NephJC |
| Katalin Susztak @KSusztak @jenniejlin @NephJC @Nephro_Sparks Love mouse models #NephJc still the only platform we can study kidney functions. I think about this quite bit especially when we discuss the different functions of the human kidney |
| Matthew Sparks, MD @Nephro_Sparks @VanessaLerma17 @NephJC that is very interesting that female mice are more susceptible to MUC1fs than male? any hypothesis and is this seen in human disease? #NephJC |
| Moran Dvela-Levitt @DvelaMoran @HecmagsMD @Nephro_Sparks @kidney_boy @Tiff_Caza @NephJC @broadinstitute indeed @HecmagsMD! He is one of our valuable collaborators! #NephJC |
| Joshua Waitzman, MD PhD @Jwaitz @NephJC It would be great to have a more streamlined process for developing patient-specific organoids so that we could quickly model and understand these mystery diseases that we treat. #NephJC |
| Nephrology Journal Club @NephJC T2a: First, are the models used in the study relevant to MKD in recapitulating the disease phenotype? Let’s take a look. #NephJC |
| Joel M. Topf, MD FACP @kidney_boy RT @Nephro_Sparks: @kidney_boy @DvelaMoran @Tiff_Caza @HecmagsMD @NephJC @broadinstitute the more families identified with mutation the quicker we can start to build cohorts and have them enroll in clinical trials. We have to start building these or we will continue to say this #NephJC #TheFutureIsNow |
| Nephrology Journal Club @NephJC T2a/1. Figure 1B shows staining of MUC1 and MUC1-fs in a kidney biopsy of a MKD patient. Figure 1D shows staining of MUC1 and MUC1-fs in the knock-in mouse model. Thoughts on fidelity of staining pattern? #NephJC https://t.co/h6TzmMpGC1 |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Jwaitz @NephJC More streamlined for reprogramming, but would also be great to have more streamlined platform for producing isogenic controls from same parent genetic background. #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @KSusztak @jenniejlin @NephJC still a missing piece to this story if the compound actually reverses pathology or improved function. I am sure they are working on this #NephJC |
| Jenn @Jentan7477 RT @NephJC: T2a/1. Figure 1B shows staining of MUC1 and MUC1-fs in a kidney biopsy of a MKD patient. Figure 1D shows staining of MUC1 and MUC1-fs in the knock-in mouse model. Thoughts on fidelity of staining pattern? #NephJC https://t.co/h6TzmMpGC1 |
| Nephrology Journal Club @NephJC T2a/2. How about the staining of MKD patient-derived iPSC kidney organoid (top) and the MKD patient-derived 2D immortalized cell line (bottom) also shown in Figure 1? #NephJC https://t.co/fWbjXtAe83 |
| Moran Dvela-Levitt @DvelaMoran @Nephro_Sparks @VanessaLerma17 @NephJC Interestingly, men are more susceptible to kidney diseases in general. in MUC1 the susceptibility is similar which suggests that there is an elevated risk for females as compare to other kidney diseases #NephJC |
| Joshua Waitzman, MD PhD @Jwaitz @Nephro_Sparks @VanessaLerma17 @NephJC Am going with "pesky hormones" on this one #NephJC https://t.co/AVFmA5nahO https://t.co/hdL86bebEH |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @KSusztak @NephJC @Nephro_Sparks I vote for a @Nephro_Sparks @KSusztak mouse model duet performance at the @NephJC #KidneyWk party. #NephJC |
| Katalin Susztak @KSusztak @Nephro_Sparks @jenniejlin @NephJC Yes that is an important piece #NephJC |
| Jenn @Jentan7477 RT @NephJC: T2a/2. How about the staining of MKD patient-derived iPSC kidney organoid (top) and the MKD patient-derived 2D immortalized cell line (bottom) also shown in Figure 1? #NephJC https://t.co/fWbjXtAe83 |
| Joel M. Topf, MD FACP @kidney_boy @NephJC The knock out mouse and MUC1 could totally switch classes and fool the teacher. #Twins #NephJC |
| Matthew Sparks, MD @Nephro_Sparks RT @DvelaMoran: @Nephro_Sparks @VanessaLerma17 @NephJC Interestingly, men are more susceptible to kidney diseases in general. in MUC1 the susceptibility is similar which suggests that there is an elevated risk for females as compare to other kidney diseases #NephJC |
| Moran Dvela-Levitt @DvelaMoran @Nephro_Sparks @KSusztak @jenniejlin @NephJC Indeed @Nephro_Sparks ! We are now trying to reverse/prevent the pathology from happening #NephJC |
 | Ben Humphreys @HumphreysLab Sorry late to #NephJC but couldn't miss conversation on this really great paper @DvelaMoran @AnnaGreka |
| Nephrology Journal Club @NephJC T2b: Gene expression profiling (RNA-seq) of N cells + P cells revealed that genes relevant to the ATF6 branch of the UPR was upregulated in MKD P cells (Fig 2A). Importance of ATF6 pathway validated in apoptotic signal when ATF6 is inhibited (Fig 2B). #NephJC https://t.co/XByBQUryik |
 | Franklin Loachamin @FranLoachamin hi #NephJC Franklin loachamin No coi . Ecuador |
| Joel M. Topf, MD FACP @kidney_boy @DvelaMoran @Nephro_Sparks @KSusztak @jenniejlin @NephJC is that an "in mice" statement? @inmice #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @NephJC staining looks good to me. You are looking a patterns here and recapitulation of human patterns. #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD Very interesting! #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @kidney_boy @DvelaMoran @KSusztak @jenniejlin @NephJC @inmice that is "in mice" for now. but important next step. #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @NephJC Nice when RNA-seq actually shows something meaningful and uncovers a relevant pathway. #NephJC |
| Nephrology Journal Club @NephJC T2b/2. Nice reviews of the different branches of the UPR for those not familiar. UPR homeostasis is important for proper protein trafficking and processing. Dysregulated UPR leads to ER stress and and integrated stress response, with potential cell death. #NephJC |
| Nephrology Journal Club @NephJC T2b/2. Here there are the link of the two reviews: https://t.co/9DUdqbBR36 https://t.co/aiF0l9VTBI #NephJC |
| Nephrology Journal Club @NephJC T2b/3. Importance of ATF6 branch of UPR was further confirmed by immunoblot of key proteins in different UPR pathways (Fig 2C). #NephJC https://t.co/GFbBu8fqEL |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @NephJC 👆ER stress important in many cardiometabolic diseases, also in podocyte (#sorrynotsorry to bring up glom in a tubule discussion) #NephJC |
| Joshua Waitzman, MD PhD @Jwaitz I think this is what everyone hopes they get when they try an RNA-seq experiment, and rarely find. #NephJC |
| Tiffany Caza @Tiff_Caza @NephJC Looks like very similar staining in mouse and human . . Good fidelity 😊. There should be no MUC1-fs staining in tubular epithelium from a healthy control or wild type mouse, is that correct? #nephjc |
| Nephrology Journal Club @NephJC T2b/4.Authors reasoned that increasing ER stress by activating other branches of UPR will also increase tubular cell injury and found that a global ER stress inducer thapsigargin(THP) increases apoptosis in P cells, leading to platform for downstream viability drug screen.#NephJC |
| Matthew Sparks, MD @Nephro_Sparks @NephJC important steps to demonstrate biologic perturbations in these pathways and important to realize that many redundancies exist. #NephJC look from multiple ways and stress the system is key |
| Tiffany Caza @Tiff_Caza @NephJC Is this being used clinically in kidney biopsies? Seems like it'd be a really useful tool #NephJC |
| Moran Dvela-Levitt @DvelaMoran @Tiff_Caza @NephJC Yes #NephJC |
| Matthew Sparks, MD @Nephro_Sparks emoji from #pathologist always a good thing #NephJC |
| Nephrology Journal Club @NephJC T2c: If MUC1-fs increases ER stress and causes disease, how would we treat? We would like to remove the mutant but not wild-type form. Maybe in the future gene therapy can be a permanent approach, and what about drugs? #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @Tiff_Caza @NephJC looks like you need to look into offering this #NephJC |
| Moran Dvela-Levitt @DvelaMoran @Tiff_Caza @NephJC Currently the only available CLIA assay is the mass spectrometer-based method developed by the @broadinstitute and available for all! #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @NephJC next slide please #NephJC |
| Nephrology Journal Club @NephJC T2d: 3713 compounds were screened at different doses to see which could remove MUC1-fs but not MUC1-wt, as assessed by staining + microscopy. 71 compounds were further screened. #NephJC https://t.co/X6W8ZczqiA |
| Nephrology Journal Club @NephJC T2d/2: The additional screens involved specificity for MUC1-fs, not repressing MUC1 mRNA (still want wild-type to be transcribed), and promoting cell viability in global ER stress (THP assay mentioned a few minutes ago). #NephJC |
| Tiffany Caza @Tiff_Caza @jenniejlin @NephJC What's the glom in a tubule reference? Non-neph here but love to play along #nephjc |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @NephJC To the authors: How difficult was it to set up the high-content screen? Very impressive! #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @NephJC what an amazing resource and would love to hear how the assay was developed. Seems like a huge undertaking to develop this and unbiased approach is so appealing. #NephJC afterparty |
| Moran Dvela-Levitt @DvelaMoran @NephJC Another major condition is the lack of toxicity! #NephJC |
| Nephrology Journal Club @NephJC T2b/3. BRD4780 passed the screening tests, including promoting viability in THP-induced ER stress, and was carried forward for further study as a potential therapeutic agent in MKD. #NephJC https://t.co/jSiOLYeU69 |
| Joshua Waitzman, MD PhD @Jwaitz @NephJC I really appreciated how the authors spelled this out in the text. Made it easy to understand what they did and why. #NephJC #OldSchool |
| Matthew Sparks, MD @Nephro_Sparks @Tiff_Caza @jenniejlin @NephJC the glom people (podocyte) just cannot deal with tubule centric talk. #LongStory #PoorMesangialCell #NephJC |
| Joel M. Topf, MD FACP @kidney_boy 3,713 compounds! This is a big lift! #NephJC |
 | 𝙼𝚊𝚝𝚝 𝙻𝚞𝚝𝚑𝚎𝚛 @DrJMLuther RT @Nephro_Sparks: Have to plug our free pre meeting that will occur Wed before the kidney meeting in DC #NephJC it will be a full day of #BasicScience #TranslationalScience Do it now!!!!!!!!!!! https://t.co/35aFfKiXCo |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Tiff_Caza @NephJC haha, no reference, I meant tubule people sometimes get offended if you bring up podocytes in a tubule-focused conversation, although I don't see any of those friends here tonight #NephJC |
| Tiffany Caza @Tiff_Caza @Nephro_Sparks @NephJC It's like every figure of this paper was a gargantuan feat. Super impressive! #researchgoals #nephjc |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD From here? https://t.co/Ro2phGpiWU #NephJC |
| Nephrology Journal Club @NephJC T2f: This result is recapitulated in human iPSC kidney organoids derived from MKD patients, establishing human and translational relevance. Note reduction of green in the “P” images of the BRD4780 row in Fig 5A. #NephJC https://t.co/TPbheMRSYr |
| Nephrology Journal Club @NephJC T2e: The results of the screen for BRD4780 was validated in vivo in the heterozygous hMUC1fs mice. Reduced green stain (MUC1-fs) in BRD4780 treated hMUC1fs mice is seen in Fig 4A, and this reduction is dose-dependent (Fig 4B-C). #NephJC https://t.co/T5mX3ThQ3h |
| Moran Dvela-Levitt @DvelaMoran @jenniejlin @NephJC That was indeed a collborative work together with our Center of development of therapeutics (CDoT) @broadinstitute and required a lot of optimization and validation #NephJC |
| Ben Humphreys @HumphreysLab Haha #Tubulointerstitium is where its at, as this MS shows :) |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @NephJC beautiful dose response #NephJC |
| Nephrology Journal Club @NephJC T2g: If MUC1-fs is trapped in the cell, where is it retained? Among organelle markers, MUC1-fs localized with TMED9 vesicles. This was seen in MKD kidney biopsy, MKD iPSC kidney organoid, P cells, and the mouse model. #NephJC https://t.co/60iy5govQB |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @NephJC @DvelaMoran how exciting was it to see this clean staining?! #NephJC |
| Ben Humphreys @HumphreysLab @kidney_boy Actually small scale in screening world - but awesome to find a lead cmpd #NephJC |
| Nephrology Journal Club @NephJC T2h: Importantly, TMED9 co-localizes with MUC1-fs but not MUC1-wt. TMED9 is cleared by BRD4780 as seen in the organoid staining experiments below. #NephJC https://t.co/et7fZEsAUZ |
| Joshua Waitzman, MD PhD @Jwaitz @HecmagsMD As a #NephJC aside, this is the coolest compound library. It's all drugs that are running out of patent time (so should be affordable if developed) and have been shelved for their primary indication. But just bc we thought we knew what something did, doesn't mean we do. |
| Joel M. Topf, MD FACP @kidney_boy Basic science #NephJC always exposes me as a poser |
| Moran Dvela-Levitt @DvelaMoran @jenniejlin @NephJC Any work with this compound resulted in super exciting results!! #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @NephJC Silly question: How did the researchers come up with the BRD4780 name? #NephJC |
| Nephrology Journal Club @NephJC T2i: Clearance of MUC1-fs happens in lysosome, not in proteasome, as seen in series of experiments involving chemical inhibitors of degradation pathways. This is important later. #NephJC |
| Nephrology Journal Club @NephJC T2j: Knocking out TMED9 in P cells using CRISPR-Cas9 genome editing led to clearance of MUC1-fs. TMED9 knockout is denoted by KO1 and KO2; there are no MUC1-fs bands in those lanes, phenocopying BRD4780. #NephJC https://t.co/9JNvpPvtSz |
| Nephrology Journal Club @NephJC T2k: So what is TMED9’s importance in all of this? It is a direct binding target of BRD4780 and thus mechanistically involved in how BRD4780 can clear MUC1-fs. Direct binding seen on thermal shift assay below. #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @NephJC that is very interesting to see Knocking down TMED9 leads to same loss of MUC-1fs. what a blot result! #NephJC |
| Moran Dvela-Levitt @DvelaMoran @Jwaitz @HecmagsMD Definitely!! the only challenge is that sometimes the target turns out to be something unexpected #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Nephro_Sparks @NephJC #dreamblot #noneedfordensitometry #NephJC |
| Ben Humphreys @HumphreysLab @kidney_boy No poser! Lead cmpds are important to validate a target and pathway, the the chemists take it but @DvelaMoran and @AnnaGreka know better than me! #NephJC |
| Nephrology Journal Club @NephJC T2l: Taken together, BRD4780 binds TMED9 to release MUC1-fs and allow it to proceed into anterograde trafficking, where it can be degraded in lysosomes. Overarching hypothesis illustrated below. Concept was applied to other proteinopathies. #NephJC https://t.co/RRqkblPiFP |
| Joel M. Topf, MD FACP @kidney_boy @HumphreysLab what did Mark Twain say about fools opening their mouths? "Better to keep your mouth shut and appear stupid than to open it and remove all doubt." #NephJC |
| Tiffany Caza @Tiff_Caza @jenniejlin @NephJC But all the compartments are special. Be a glomerulotubulointerstitialvasculo-phile! #nephjc |
| Ben Humphreys @HumphreysLab @HecmagsMD @NephJC I am guessing BRD is Broad Inst? #NephJC @DvelaMoran |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @DvelaMoran @Jwaitz Can wait to hear more about these “unexpected” things?!?! Your team struck gold! #NephJC |
| Joshua Waitzman, MD PhD @Jwaitz These thermal shift assays warmed this biochemist's heart. #NephJC #dadjoke https://t.co/O9aVQW1Xwg |
| Matthew Sparks, MD @Nephro_Sparks RT @NephJC: T2l: T2l: Taken together, BRD4780 binds TMED9 to release MUC1-fs and allow it to proceed into anterograde trafficking, where it can be degraded in lysosomes. Overarching hypothesis illustrated below. Concept was applied to other proteinopathies. #NephJC https://t.co/RRqkblPiFP |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Tiff_Caza @NephJC #nephronpower #beanephronpowerranger #NephJC |
| Nephrology Journal Club @NephJC Time for the last ten minute: T3: Now, on to discussion. #NephJC |
| Matthew Sparks, MD @Nephro_Sparks @Jwaitz too funny. Josh going strong to the end of the paper #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Jwaitz oh right, you're a dad #dadjoke on #NephJC |
| Nephrology Journal Club @NephJC T3a: What were your favorite strengths of the paper? #NephJC |
| Matthew Sparks, MD @Nephro_Sparks What are the next steps to showing how BRD4780 interacts with TMED9? also TMED9 expressed in many many cell types.... what are implications of long term Rx with BRD? #NephJC |
| Nephrology Journal Club @NephJC T3b: What are some limitations, if any, to bringing BRD4780 or even TMED9 CRISPR forward for clinical translation? #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @NephJC Multi-pronged approach with models (discovery and validation) and leveraging unbiased methods to drill down on mechanism #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Nephro_Sparks CRISPR TMED9 via cell-specific delivery? #NephJC |
| Joel M. Topf, MD FACP @kidney_boy I want this to happen so bad. I want a disease that we didn't bother diagnosing because it was subtle and hard and untreatable to suddenly have a treatment. What a win for nephrology. What a win for out patients. What a win for Science! #nephjc |
| Tiffany Caza @Tiff_Caza @jenniejlin @NephJC @DvelaMoran Such beautiful confocal images! The authors are rock stars. #researchgoals #nephjc |
| Matthew Sparks, MD @Nephro_Sparks @NephJC need to show that long term Rx in mice with MUC1-fs leads to reduced pathology and/or improvement in kidney function. Toxicity studies will also be needed #NephJC |
| Joshua Waitzman, MD PhD @Jwaitz @jenniejlin @NephJC So much this. It's all mechanism here, which is why it's a really fun paper to read. #NephJC |
| Moran Dvela-Levitt @DvelaMoran @Nephro_Sparks Definitely, this opens many new doors. and one of the clear next steps would be to test for the compound toxicology thoroughly #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Tiff_Caza @NephJC @DvelaMoran #iknowright The only stain I can get to work consistently for confocal images is DAPI 🤣😭#NephJC |
| Nephrology Journal Club @NephJC T3c: @DvelaMoran : What's in the pipeline, and what is the next step in the development of BRD4780? #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD Yes to everything! #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Nephro_Sparks @NephJC Toxicity for sure. What are kidney organoid toxicity screen readouts at the moment? #NephJC |
| Joshua Waitzman, MD PhD @Jwaitz @NephJC @DvelaMoran Do you guys have a pharma partner already lined up? Or is there more work you feel like you need to do before you can get industry interest? #NephJC |
| Michelle Rheault @rheault_m @kidney_boy And then the Pharma company that owns the therapy will do everything they can to make sure the diagnosis is made. #nephJC |
| Nephrology Journal Club @NephJC T3d: Any final thoughts or comments? #NephJC |
| Sami Alasfar, MD, MPH, FASN @SamiAlasfar Nicely said #NephJC |
| Joel M. Topf, MD FACP @kidney_boy @rheault_m Bring it on! Where's the speaker bureau? Count me in! #NephJC |
| Tiffany Caza @Tiff_Caza @NephJC It's all strong! Well designed experiments, mechanistic detail, and a treatment target. What else could you want? 😁. #nephjc |
| Joshua Waitzman, MD PhD @Jwaitz @jenniejlin @Nephro_Sparks @NephJC Is there a sense from regulators that they're ready to accept organoid/organ-on-a-chip readouts of toxicity as a surrogate for Phase II trials? #NephJC |
| Matthew Sparks, MD @Nephro_Sparks want to thank @DvelaMoran for joining and sharing this important research. Happy to cover this on #NephJC and looking forward to further developments. Thanks to @AnnaGreka and the entire team. #NephForward |
| Moran Dvela-Levitt @DvelaMoran @NephJC Definitely to do toxicology studies in order to move on with this compound to the clinic, as well as testing the effect of the compound on reversing/preventing the pathology. and a deeper understanding of the mechanism of the compound #NephJC |
| Anna Greka @AnnaGreka @Jwaitz @NephJC @DvelaMoran Lots of interest and lots of activity! We still have a lot to do to bring a safe drug to the clinic, with the right partners! Working on it! #nephjc |
| Nephrology Journal Club @NephJC Thank you, everyone, for your participation in tonight’s discussion! #NephJC |
| Nephrology Journal Club @NephJC Join us for our next #NephJC September 24th and 25th! #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD @Nephro_Sparks @DvelaMoran @AnnaGreka Indeed. THANK YOU. #NephJC |
| Matthew Sparks, MD @Nephro_Sparks also great job to the entire #NephJC team and #NSMC for pulling off another #basicscience edition @VanessaLerma17 summary @SusieLMurray editorial & @MarvinGonzlez16 hosting also huge shoutout to @jenniejlin and @HecmagsMD for edits massive team effort to pull this off |
| Joshua Waitzman, MD PhD @Jwaitz @AnnaGreka @NephJC @DvelaMoran We know it's in good hands! Thanks @annagreka #NephJC |
| Jennie Lin 👩🏻⚕️🧬 @jenniejlin @Jwaitz @Nephro_Sparks @NephJC doubtful as a surrogate without proper vascularization, but maybe the toxicity screen can filter out compounds that will cause mito damage for example or apoptosis #NephJC |
| Marvin Gonzalez. MD, Ph.D @MarvinGonzlez16 RT @Nephro_Sparks: also great job to the entire #NephJC team and #NSMC for pulling off another #basicscience edition @VanessaLerma17 summary @SusieLMurray editorial & @MarvinGonzlez16 hosting also huge shoutout to @jenniejlin and @HecmagsMD for edits massive team effort to pull this off |
| Nephrology Journal Club @NephJC Goodnight all! #NephJC |
| Moran Dvela-Levitt @DvelaMoran @Nephro_Sparks @AnnaGreka Thank you @Nephro_Sparks and @NephJC for hosting us! it was a great pleasure! #NephJC |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD RT @AnnaGreka: @Jwaitz @NephJC @DvelaMoran Lots of interest and lots of activity! We still have a lot to do to bring a safe drug to the clinic, with the right partners! Working on it! #nephjc |
| Joel M. Topf, MD FACP @kidney_boy Bravo to the whole #NephJC team and all of the basic science nerds for coming out and schooling us other type of nerds about this. Great basic science chat. Love this shit! |
| Matthew Sparks, MD @Nephro_Sparks The #MUC1 numbers: 336 Tweets. 54 Participants. 2,057,771 Impressions. September 2nd 2019. More #NephJC 📊 here https://t.co/WImVo41kV5 via @symplur |
| Sami Alasfar, MD, MPH, FASN @SamiAlasfar thank you #NephJC I am not basic scientist but I was certainly enlightened about this topic |
| Joshua Waitzman, MD PhD @Jwaitz @Nephro_Sparks @VanessaLerma17 @SusieLMurray @MarvinGonzlez16 @jenniejlin @HecmagsMD Nice job team #NephJC! I love that you're building out the wheelhouse for the medicines of the future that we'll get to prescribe to our patients. |
| Hector Madariaga, MD FASN 🇲🇽 @HecmagsMD RT @kidney_boy: Bravo to the whole #NephJC team and all of the basic science nerds for coming out and schooling us other type of nerds about this. Great basic science chat. Love this shit! |
 | ADOLFO LEON CASTRO NAVAS @ADOLFOLEONCAST4 RT @ssfarouk: From the #NephJC summary MKD = MUC-1 kidney disease Mutations in the MUC1 gene (on chromosome 1q21) have been identified to cause progressive tubulointerstitial kidney disease inherited in an autosomal dominant fashion and thus now termed, MKD |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @Nephro_Sparks: The #MUC1 numbers: The #MUC1 numbers: 336 Tweets. 54 Participants. 2,057,771 Impressions. September 2nd 2019. More #NephJC 📊 here https://t.co/WImVo41kV5 via @symplur |
| Marvin Gonzalez. MD, Ph.D @MarvinGonzlez16 RT @Jwaitz: @Nephro_Sparks @VanessaLerma17 @SusieLMurray @MarvinGonzlez16 @jenniejlin @HecmagsMD Nice job team #NephJC! I love that you're building out the wheelhouse for the medicines of the future that we'll get to prescribe to our patients. |
| Swapnil Hiremath @hswapnil@bsky.social @hswapnil RT @Nephro_Sparks: also great job to the entire #NephJC team and #NSMC for pulling off another #basicscience edition @VanessaLerma17 summary @SusieLMurray editorial & @MarvinGonzlez16 hosting also huge shoutout to @jenniejlin and @HecmagsMD for edits massive team effort to pull this off |
| Moran Dvela-Levitt @DvelaMoran @kidney_boy This is actually @AnnaGreka lab's driving concept! To take a rare disease find its mechanism, find a treatment and to try and apply it to other complex disorders! #NephJC |
| Marvin Gonzalez. MD, Ph.D @MarvinGonzlez16 @Nephro_Sparks @symplur This #NephJC was increible... I learnt many things on this topic. |
| Tiffany Caza @Tiff_Caza RT @kidney_boy: I want this to happen so bad. I want a disease that we didn't bother diagnosing because it was subtle and hard and untreatable to suddenly have a treatment. What a win for nephrology. What a win for out patients. What a win for Science! #nephjc |
 | Paresh Jadav, MD, FASN @jadav_md My first day on twitter and was a blast! Never knew that social media could have such an enlightening experience!! #nephjc |
| Marvin Gonzalez. MD, Ph.D @MarvinGonzlez16 @DvelaMoran @Nephro_Sparks She was very active in the chat and tried to respond almost all the questions #NephJC @DvelaMoran Great job job Dr Dvela-Levitt |
#NephJC content from Twitter.